1LKX

MOTOR DOMAIN OF MYOE, A CLASS-I MYOSIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	80
Detector technology	CCD
Collection date	2000-01-01
Detector	MARRESEARCH
Wavelength(s)	0.8424
Spacegroup name	P 1 21 1
Unit cell lengths	51.818, 143.667, 236.050
Unit cell angles	90.00, 94.86, 90.00

Refinement procedure

Resolution	50.000 - 3.000
Rwork	0.228
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	21.220 *
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000	3.000 *
Rmerge	0.195 *	0.280 *
Number of reflections	54968
Completeness [%]	79.7 *	57.4 *
Redundancy	3.8 *	2.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	16 or 17 *	11% PEG 8K, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	7.2	16 or 17 *	11% PEG 8K, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 289K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	11 (%(w/v))
2	1	reservoir		170 (mM)
3	1	reservoir	HEPES-NaOH	50 (mM)	pH7.2
4	1	reservoir		5 (mM)
5	1	reservoir	dithiothreitol	5 (mM)
6	1	reservoir	EGTA	0.5 (mM)
7	1	reservoir	MPD	2 (%)
8	1	drop	ADP	0.2 (mM)
9	1	drop	vanadate	0.18 (mM)