1LKT

CRYSTAL STRUCTURE OF THE HEAD-BINDING DOMAIN OF PHAGE P22 TAILSPIKE PROTEIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1996-04
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	57.300, 82.100, 73.800
Unit cell angles	90.00, 90.90, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.186 *
Rwork	0.199
Structure solution method	MIR
RMSD bond length	0.009 *
RMSD bond angle	1.490 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.650
High resolution limit [Å]	2.600	2.600
Rmerge	0.076	0.298
Total number of observations	48766 *
Number of reflections	20822
Completeness [%]	96.5	72.2
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.6		20% PEG 8K, 0.2 M MGCL2, 0.1 M BIS-TRIS, PH 6.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	20 (%(w/v))
2	1	reservoir		0.2 (M)
3	1	reservoir	Bis-Tris-HCl	0.1 (M)