1LKL

HUMAN P56-LCK TYROSINE KINASE SH2 DOMAIN IN COMPLEX WITH THE PHOSPHOTYROSYL PEPTIDE AC-PTYR-GLU-GLU-GLY (PYEEG PEPTIDE)

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	81.850, 45.150, 26.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.189
Rwork	0.189
RMSD bond length	0.009
RMSD bond angle	1.500
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.043 *
Total number of observations	34222 *
Number of reflections	9668 *
Completeness [%]	98.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	SH2 domain	35 (mg/ml)
2	1	drop	peptide	42 (mM)
3	1	drop	BES	40 (mM)
4	1	reservoir	PEG5000 monomethyl ether	30-32 (%(w/v))
5	1	reservoir	BES	100 (mM)
6	1	reservoir	ammonium sulfate	100-200 (mM)