1LKK

HUMAN P56-LCK TYROSINE KINASE SH2 DOMAIN IN COMPLEX WITH THE PHOSPHOTYROSYL PEPTIDE AC-PTYR-GLU-GLU-ILE (PYEEI PEPTIDE)

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	81.840, 45.040, 26.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.000
R-factor	0.133
RMSD bond length	0.012
RMSD bond angle	1.700 *
Phasing software	SHELXL-93
Refinement software	SHELXL-93

Data quality characteristics

	Overall
High resolution limit [Å]	1.000 *
Rmerge	0.070 *
Total number of observations	170160 *
Number of reflections	48786 *
Completeness [%]	91.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	SH2 domain	35 (mg/ml)
2	1	drop	peptide	42 (mM)
3	1	drop	BES	40 (mM)