1LKE
ENGINEERED LIPOCALIN DIGA16 IN COMPLEX WITH DIGOXIGENIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-03 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.052, 63.988, 39.452 |
| Unit cell angles | 90.00, 91.53, 90.00 |
Refinement procedure
| Resolution | 48.800 - 1.900 |
| R-factor | 0.18808 |
| Rwork | 0.185 |
| R-free | 0.24753 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1bbp |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.541 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 14294 | |
| <I/σ(I)> | 22 | 6 |
| Completeness [%] | 96.7 | 96.1 |
| Redundancy | 2.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1-M HEPES, 10% ISOPROPANOL, 19% PEG 4000, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
