1LJY

Crystal Structure of a Novel Regulatory 40 kDa Mammary Gland Protein (MGP-40) secreted during Involution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2001-12-26
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.950, 65.890, 107.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.900
R-factor	0.18
Rwork	0.181
R-free	0.23500 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009 *
RMSD bond angle	1.700 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.114 *	0.296 *
Number of reflections	10394
<I/σ(I)>	7
Completeness [%]	99.3	99
Redundancy	5.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	8 *	277	12%Ethanol, 25 mMTris-HCl, pH 8.5, MICRODIALYSIS, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	Tris-HCl	10 (mM)
3	1	2	ethanol	19 (%(v/v))	pH8.0