1LJP
Crystal Structure of beta-Cinnamomin Elicitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.850, 36.910, 43.930 |
| Unit cell angles | 77.64, 86.67, 79.55 |
Refinement procedure
| Resolution | 21.400 - 1.800 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1beo |
| RMSD bond length | 0.004 |
| RMSD bond angle | 19.092 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.400 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.036 | 0.121 |
| Total number of observations | 61726 * | |
| Number of reflections | 16756 | |
| <I/σ(I)> | 31.2 | 10.5 |
| Completeness [%] | 94.3 | 90.3 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 3350, butanol, ammonium sulfate, Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
