1LJN
Crystal Structure of Turkey Egg Lysozyme Complex with Di-N-acetylchitobiose at 1.19A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 286 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-11-19 |
| Detector | ENRAF-NONIUS FAST |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.200, 33.290, 46.210 |
| Unit cell angles | 90.00, 109.94, 90.00 |
Refinement procedure
| Resolution | 15.800 - 1.190 |
| R-factor | 0.104 |
| Rwork | 0.103 |
| R-free | 0.13800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lzy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.020 |
| Data reduction software | MERGEF |
| Data scaling software | MERGEF |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.800 | 1.210 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.052 | 0.206 |
| Total number of observations | 103984 * | |
| Number of reflections | 32432 * | |
| Completeness [%] | 92.0 * | 49.1 |
| Redundancy | 3.21 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.2 | 293 | Harata, K., (1993) Acta Crystallogr., D49, 497. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | aqueous 1-propanol solution | 10 (%) | |
| 2 | 1 | 1 | TEL | 100 (mg) | |
| 3 | 1 | 1 | ammonium sulfate | 3 (M) |
