1LJK

CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN IN PRESENCE OF 15% TREHALOSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	28.066, 62.936, 60.406
Unit cell angles	90.00, 90.68, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.171
Rwork	0.171
R-free	0.21900
RMSD bond length	0.005
RMSD bond angle	23.200 *
Data scaling software	SCALEPACK
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.080 *	0.247 *
Total number of observations	45545 *
Number of reflections	12088 *	1194 *
Completeness [%]	97.6 *	96 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293	Sodium Acetate(0.1M)-Buffer, Sodium Nitrate (0.44M)-Precipitant, Trehalose(0.44M) - Additive, pH 4.6, LIQUID DIFFUSION at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium acetate	0.1 (M)	pH4.6
2	1	1	trehalose	15 (%)