1LJG

CRYSTAL STRUCTURE OF MONOCLINIC LYSOZYME GROWN IN PRESENCE OF 5% GLYCEROL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	27.857, 62.714, 60.019
Unit cell angles	90.00, 90.42, 90.00

Refinement procedure

Resolution	30.000 - 1.950 *
R-factor	0.205
Rwork	0.205
R-free	0.24300
RMSD bond length	0.005
RMSD bond angle	23.300 *
Data scaling software	SCALEPACK
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.046 *	0.120 *
Total number of observations	61157 *
Number of reflections	15695 *	1520 *
Completeness [%]	95.8 *	93.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	293	Sodium Acetate(0.1M)-Buffer, Sodium Nitrate (0.44M)-Precipitant, glycerol(0.54M) - Additive; pH 4.6, LIQUID DIFFUSION at 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium acetate	0.1 (M)	pH4.6
2	1	1	glycerol	5 (%)