1LJ9
The crystal structure of the transcriptional regulator SlyA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-21 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9793, 0.9795, 0.95354 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.512, 48.429, 125.999 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.600 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.27500 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.398 |
| Number of reflections | 32997 | |
| <I/σ(I)> | 23.26 | 2.1 |
| Completeness [%] | 91.1 | 79.8 |
| Redundancy | 6.295 | 4.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 283 * | PEG 3350, 22%, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | pH7.4 |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | PEG3350 | 30 (%) | |
| 5 | 1 | reservoir | glycerol | 20 (%) | |
| 6 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.6 |
