1LJ7
Crystal structure of calcium-depleted human C-reactive protein from perfectly twinned data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 298 |
| Detector technology | FILM |
| Collection date | 1989-01-01 |
| Detector | CEA |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 43 |
| Unit cell lengths | 102.310, 102.310, 309.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.150 |
| R-factor | 0.186 * |
| Rwork | 0.186 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Amended pentamer from PDB ENTRY 1GNH |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.600 | 3.250 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.114 | 0.276 |
| Number of reflections | 26903 | |
| <I/σ(I)> | 5.4 | 2.4 |
| Completeness [%] | 92.1 | 55.8 |
| Redundancy | 5.3 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | DeLucas, L.J., (1987) J. Mol. Biol., 196, 741. * |
