1LJ1
Crystal structure of Q363F/R402A mutant flavocytochrome c3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8459 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.971, 88.280, 90.087 |
| Unit cell angles | 90.00, 103.89, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| Rwork | 0.161 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qjd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.226 |
| Total number of observations | 412535 * | |
| Number of reflections | 75611 | |
| <I/σ(I)> | 9.2 | 2.49 |
| Completeness [%] | 94.5 | 92.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 4 * | TrisHCl, PEG8000, sodium chloride, sodium fumarate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.8-8.5 |
| 2 | 1 | reservoir | 80 (mM) | ||
| 3 | 1 | reservoir | PEG8000 | 16-19 (%) | |
| 4 | 1 | reservoir | PEG8000 | 10 (mM) | |
| 5 | 1 | drop | protein | 6 (mg/ml) | |
| 6 | 1 | drop | Tris-HCl | 10 (mM) | pH8.5 |
