1LJ0

Structure of quintuple mutant of the rat outer mitocondrial cytochrome b5.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-02-08
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	39.753, 50.854, 167.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.000 - 2.000
Rwork	0.207
R-free	0.25100
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1icc
RMSD bond length	0.015
RMSD bond angle	1.610
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	0.370
Number of reflections	23729
<I/σ(I)>	22	3.2
Completeness [%]	99.2	94.7
Redundancy	5	3.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	278	PEG 8000, MG ACETATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	PEG	32 (%(w/v))
3	1	reservoir	magnesium acetate	0.2 (M)
4	1	reservoir	PIPES	0.1 (M)	pH6.5