1LIT

HUMAN LITHOSTATHINE

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Detector technology	IMAGE PLATE
Collection date	1994
Detector	MARRESEARCH
Spacegroup name	P 65
Unit cell lengths	47.190, 47.190, 108.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.550
R-factor	0.186 *
Rwork	0.180
R-free	0.24400 *
RMSD bond length	0.010 *
RMSD bond angle	2.100
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Rmerge	0.042
Number of reflections	19356
Completeness [%]	98.2
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *	Pignol, D., (1995) Proteins: Struct.,Funct., Genet., 23, 604. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	100 (mM)
2	1	reservoir	PEG4000	12 (%(w/v))
3	1	drop	protein	7 (mg/ml)
4	1	drop	sodium acetate	50 (mM)
5	1	drop	PEG4000	6 (%(w/v))