1LIN
CALMODULIN COMPLEXED WITH TRIFLUOPERAZINE (1:4 COMPLEX)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Detector technology | IMAGE PLATE |
| Collection date | 1989-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 40.660, 40.660, 177.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.483 |
| Data reduction software | MADNES |
| Phasing software | X-PLOR (2.1) |
| Refinement software | X-PLOR (2.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.100 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.061 |
| Number of reflections | 12001 |
| Completeness [%] | 97.6 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 * | ||
| 1 | Vapor diffusion * | 5.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.6 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 10.5 (%) | |
| 3 | 1 | drop | cacodylate | 8 (mM) | |
| 4 | 1 | drop | calcium chloride | 16 (mM) | |
| 5 | 1 | drop | TFP | 5.4 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 22 (%) | |
| 7 | 1 | reservoir | cacodylate | 10 (mM) | |
| 8 | 1 | reservoir | calcium chloride | 10 (mM) |
