1LIL

BENCE JONES PROTEIN CLE, A LAMBDA III IMMUNOGLOBULIN LIGHT-CHAIN DIMER

Experimental procedure

Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1989
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 21 21 21
Unit cell lengths	113.170, 72.540, 49.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.650
R-factor	0.2
Rwork	0.190
R-free	0.34000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	0.039
Data reduction software	SCALING
Data scaling software	SCALING
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.500 *
High resolution limit [Å]	2.650
Rmerge	0.047
Total number of observations	55038 *
Number of reflections	11373
Completeness [%]	92.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7		Stevens, F.J., (1981) J.Mol.Biol., 147, 179. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	2	ammonium sulfate	3.0 (M)
3	1	2	ammonium hydroxide	1.8 (M)