Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 167.800, 47.100, 44.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.710 * |
| R-factor | 0.178 * |
| Rwork | 0.189 |
| R-free | 0.22900 * |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 1.870 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.760 |
| High resolution limit [Å] | 1.710 * | 1.720 |
| Rmerge | 0.059 | 0.285 * |
| Total number of observations | 132352 * | |
| Number of reflections | 38408 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 99.0 * | 99 * |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | ammonium sulphate, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | adenosine | 5 (mM) | |
| 3 | 1 | reservoir | 1.1 (M) | ||
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | sodium cacodylate | 50 (mM) | pH6.5 |
