1LIA

CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN FROM POLYSIPHONIA AT 2.8 A RESOLUTION

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-03-15
Detector	SIEMENS-NICOLET X200B
Spacegroup name	H 3
Unit cell lengths	189.800, 189.800, 60.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.18
Rwork	0.180
R-free	0.18200
RMSD bond length	0.020
RMSD bond angle	22.780 *
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.730
Rmerge	0.042
Number of reflections	21280
Completeness [%]	93.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium phosphate	0.05 (M)
2	1	drop	ammonium sulfate	3 (%(w/v))	or PEG6000
3	1	drop	protein	10 (mg/ml)
4	1	reservoir	sodium phosphate	0.05 (M)
5	1	reservoir	ammonium sulfate	8 (%)
6	1	reservoir		3 (%(w/v))