1LHP

Crystal Structure of Pyridoxal Kinase from Sheep Brain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	298
Detector technology	DIFFRACTOMETER
Collection date	2000-11-19
Detector	WEISSENBERG
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	59.800, 94.400, 128.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.196
Rwork	0.195
R-free	0.22400
Structure solution method	MIR
RMSD bond length	0.008 *
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.066	0.353
Total number of observations	182984 *
Number of reflections	42820
<I/σ(I)>	20.4	4.1
Completeness [%]	99.1	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	17 *	sodium citrate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium citrate	0.1 (M)	pH5.8
3	1	reservoir	sodium citrate	1.1 (M)	pH5.8