1LHP
Crystal Structure of Pyridoxal Kinase from Sheep Brain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2000-11-19 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.800, 94.400, 128.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.22400 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.066 | 0.353 |
| Total number of observations | 182984 * | |
| Number of reflections | 42820 | |
| <I/σ(I)> | 20.4 | 4.1 |
| Completeness [%] | 99.1 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 17 * | sodium citrate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium citrate | 0.1 (M) | pH5.8 |
| 3 | 1 | reservoir | sodium citrate | 1.1 (M) | pH5.8 |
