1LHI

ROLE OF PROLINE RESIDUES IN HUMAN LYSOZYME STABILITY: A SCANNING CALORIMETRIC STUDY COMBINED WITH X-RAY STRUCTURE ANALYSIS OF PROLINE MUTANTS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	56.590, 60.710, 33.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.800
R-factor	0.156
RMSD bond length	0.017
RMSD bond angle	0.037 *
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.050 *
Total number of observations	25987 *
Number of reflections	9952 *
Completeness [%]	87.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.8 *	13 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1		2.5 (M)	precipitant
3	1	1	sodium phosphate	30 (mM)