1LGY

LIPASE II FROM RHIZOPUS NIVEUS

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1992-01-16
Detector	RIGAKU
Spacegroup name	P 43
Unit cell lengths	83.700, 83.700, 137.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.186 *
Rwork	0.186
RMSD bond length	0.016
Data reduction software	RIGAKU
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.089
Number of reflections	53656
Completeness [%]	78.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	14-18 (%)
2	1	reservoir	HEPES	0.1 (M)
3	1	drop	protein	18 (mg/ml)