1LGN
DECAMERIC DAMP COMPLEX OF HUMAN SERUM AMYLOID P COMPONENT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 190.030, 190.030, 119.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1SAC WITHOUT WATER MOLECULES AND ACETATE IONS ALL B-FACTORS SET TO 40 A**2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.068 * | 0.443 |
| Total number of observations | 366263 * | |
| Number of reflections | 53133 * | |
| <I/σ(I)> | 6.8 | 1.4 |
| Completeness [%] | 98.0 | 97.5 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 140 (mM) | ||
| 4 | 1 | drop | dAMP | 20 (mM) | |
| 5 | 1 | drop | 0.02 (%(w/v)) | ||
| 6 | 1 | reservoir | bis-Tris propane-HCl | 0.1 (M) | |
| 7 | 1 | reservoir | PEG4000 | 14 (%(w/v)) | |
| 8 | 1 | reservoir | 20 (mM) |
