1LFQ

OXY HEMOGLOBIN (93% RELATIVE HUMIDITY)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 41 21 2
Unit cell lengths	54.200, 54.200, 195.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.197
Rwork	0.197
R-free	0.25800
RMSD bond length	0.011
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.129	0.370
<I/σ(I)>	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION	6.7	298	phosphate buffer, pH 6.7, LIQUID DIFFUSION at 298K