1LFM
CRYSTAL STRUCTURE OF COBALT(III)-SUBSTITUTED CYTOCHROME C (TUNA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 74.175, 74.175, 35.561 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.690 - 1.500 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22000 |
| Structure solution method | rigid body refinement |
| Starting model (for MR) | 3cyt |
| RMSD bond length | 0.018 |
| RMSD bond angle | 22.500 * |
| Data reduction software | XFIT |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.690 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.073 * | |
| Number of reflections | 27926 | |
| <I/σ(I)> | 17.3 | 3.2 |
| Completeness [%] | 89.2 | 88.4 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 298 | (NH4)2SO4, NaCl, Na3PO4, pH 6.4, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 70-75 (%sat) | |
| 3 | 1 | reservoir | 0.75 (M) | ||
| 4 | 1 | reservoir | NaPi | 0.1 (M) | pH6.5 |
