1LFI

METAL SUBSTITUTION IN TRANSFERRINS: THE CRYSTAL STRUCTURE OF HUMAN COPPER-LACTOFERRIN AT 2.1 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	155.900, 97.000, 56.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 * - 2.100
R-factor	0.199
RMSD bond length	0.017
RMSD bond angle	0.063 *
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.089 *
Number of reflections	46683 *	4081 *
Completeness [%]		33 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *		4 *	taken from Norris, G.E. et al (1989). J. Mol. Biol., 209, 329-331. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium pshosphate	0.01 (M)
2	1	1	methanol	10 (%(v/v))
3	1	2	protein	30-40 (mg/ml)