1LFH

MOLECULAR REPLACEMENT SOLUTION OF THE STRUCTURE OF APOLACTOFERRIN, A PROTEIN DISPLAYING LARGE-SCALE CONFORMATIONAL CHANGE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	152.090, 94.580, 55.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.800
R-factor	0.213
RMSD bond length	0.020
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.800 *
Rmerge	0.090 *
Total number of observations	19413 *
Number of reflections	17783 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.8 *	4 *	taken from Baker, E.N. et al (1977). J. Mol. Biol., 111, 207-210. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	2-5 (%)
2	1	2	phosphate	0.01 (M)
3	1	2	ethanol	10 (%(v/v))