1LF7
Crystal Structure of Human Complement Protein C8gamma at 1.2 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 94 |
| Detector technology | PIXEL |
| Collection date | 1999-06-26 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.448, 58.993, 72.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.030 - 1.200 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.22900 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.043 | |
| Number of reflections | 53030 | |
| Completeness [%] | 92.2 | 63.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | 4 * | PEG 4000, sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | imidazole | 25 (mM) | |
| 2 | 1 | drop | 150 (mM) | pH7.2 | |
| 3 | 1 | drop | protein | 0.3-0.5 (mg/ml) | |
| 4 | 1 | reservoir | PEG4000 | 28-36 (%) | |
| 5 | 1 | reservoir | sodium citrate | 0.1 (M) | pH4.0 |
