1LF6
CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.01 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.186, 102.513, 164.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.100 |
| Rwork | 0.194 |
| R-free | 0.23000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.035 | 0.070 |
| Total number of observations | 446637 * | |
| Number of reflections | 76199 | |
| <I/σ(I)> | 38.5 | |
| Completeness [%] | 90.0 | 82 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | PEG 3350, Tris-HCl, Lithium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 24K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 2 | 1 | reservoir | PEG3350 | 14-16 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.0 |
| 4 | 1 | reservoir | 200 (mM) |
