1LEX
STRUCTURE OF A DICATIONIC MONOIMIDAZOLE LEXITROPSIN BOUND TO DNA (ORIENTATION 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 248 |
| Detector technology | DIFFRACTOMETER |
| Detector | RIGAKU AFC-5R |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.030, 39.260, 66.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.250 |
| R-factor | 0.165 |
| R-free | 0.24800 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.066 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.250 | 2.250 * |
| Completeness [%] | 85.0 * | 70 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 * | standing drop * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MG ACETATE | ||
| 4 | 1 | 1 | SPERMINE_HCL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA duplex | 0.43 (mM) | |
| 2 | 1 | drop | magnesium acetate | 6.0 (mM) | |
| 3 | 1 | drop | spermine hydrochloride | 0.2 (mM) | |
| 4 | 1 | drop | drug | 0.73 (mM) | |
| 5 | 1 | drop | MPD | 8.8 (%(w/v)) | |
| 6 | 1 | reservoir | 40 (%(w/v)) |
