1LES

LENTIL LECTIN COMPLEXED WITH SUCROSE

Experimental procedure

Detector technology	DIFFRACTOMETER
Collection date	1994-06-24
Detector	ENRAF-NONIUS FAST
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	50.030, 124.800, 50.020
Unit cell angles	90.00, 111.80, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.188
Rwork	0.188
RMSD bond length	0.020
RMSD bond angle	28.840 *
Data reduction software	MADNES
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.073	0.295 *
Total number of observations	114331 *
Number of reflections	43108
Completeness [%]	69.9	75.1 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.25 (mg/ml)
2	1	drop	cacodylate	50 (mM)
3	1	drop	sucrose	25 (mM)
4	1	drop	MPD	20 (%(v/v))
5	1	reservoir	MPD	40 (%(v/v))
6	1	reservoir	cacodylate	100 (mM)