1LEP
THREE-DIMENSIONAL STRUCTURE OF THE IMMUNODOMINANT HEAT-SHOCK PROTEIN CHAPERONIN-10 OF MYCOBACTERIUM LEPRAE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1995 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 112.900, 129.000, 109.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.500 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.38000 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.800 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 3.500 * | 3.500 * |
| Rmerge | 0.088 | |
| Completeness [%] | 98.0 * | 90 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 5 (mM) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | reservoir | HEPES | 100 (mM) | |
| 5 | 1 | reservoir | PEG400 | 35 (%(v/v)) | |
| 6 | 1 | reservoir | 150 (mM) |
