1LEO

P100S NUCLEOSIDE DIPHOSPHATE KINASE

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE
Synchrotron site	LURE
Collection date	1995-06
Detector	MARRESEARCH
Spacegroup name	P 63 2 2
Unit cell lengths	75.044, 75.044, 105.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.600
R-factor	0.193
Rwork	0.193
RMSD bond length	0.018
RMSD bond angle	2.030
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.600 *
Rmerge	0.109
Total number of observations	88483 *
Number of reflections	5263 *
Completeness [%]	95.6
Redundancy	16.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)
2	1	drop		20 (mM)
3	1	drop	PEG3350	9-11 (%)
4	1	drop	protein	6 (mg/ml)
5	1	reservoir	PEG3350	18-22 (%)
6	1	reservoir	Tris-HCl	50 (mM)