1LEG

Crystal Structure of H-2Kb bound to the dEV8 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	170
Detector technology	IMAGE PLATE
Collection date	1997-12-06
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	135.687, 87.686, 45.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.750
R-factor	0.206 *
Rwork	0.217
R-free	0.25100 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.620
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.058 *	0.682 *
Total number of observations	339981 *
Number of reflections	56955
Completeness [%]	86.5	89.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.9 *	4 *	sodium potassium phosphate, pH 6.8, VAPOR DIFFUSION, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-acetate	0.1 (M)	pH6.9
2	1	reservoir	PEG4000	12.5 (%)
3	1	reservoir	glycerol	20 (%)