1LDN

STRUCTURE OF A TERNARY COMPLEX OF AN ALLOSTERIC LACTATE DEHYDROGENASE FROM BACILLUS STEAROTHERMOPHILUS AT 2.5 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	84.900, 118.200, 135.500
Unit cell angles	90.00, 96.07, 90.00

Refinement procedure

Resolution	10.000 * - 2.500
R-factor	0.157
RMSD bond length	0.011
RMSD bond angle	0.045
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.097 *
Total number of observations	99326 *
Number of reflections	62506 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		taken from Wigley, B., et al. (1988). J. Mol. Biol., 204, 1041-1044. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	PEG6000	12 (%)	or PEG8000
3	1	drop	trisethanolamine	10 (mM)