1LDI

CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITHOUT SUBSTRATE GLYCEROL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-07
Detector	ADSC QUANTUM
Spacegroup name	I 4 2 2
Unit cell lengths	96.053, 96.053, 184.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.229 *
Rwork	0.229
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fx8
RMSD bond length	0.007
RMSD bond angle	1.260
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700	2.700
Rmerge	0.081	0.322
Total number of observations	64951 *
Number of reflections	10918
<I/σ(I)>	14
Completeness [%]	87.5	83.2
Redundancy	5.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.9 *	298	GLPF at 15-20 mg/ml, 100 mM Bicine, 28% (w/v) PEG 2000, 300mM MgCl2, 5 mMDTT, 15 % (v/v) xylose, 35 mM N-octyl-beta-D-glucoside, pH 9.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15-20 (mg/ml)
2	1	2	PEG2000	28 (%(w/v))
3	1	2	bicine	100 (mM)
4	1	2	glycerol	15 (%(v/v))
5	1	2	n-octyl-beta-D-glucoside	35 (mM)
6	1	2		300 (mM)
7	1	2	dithiothreitol	5 (mM)