1LCZ

streptavidin-BCAP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2001-02-13
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	I 2 2 2
Unit cell lengths	47.483, 94.925, 105.447
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.950
Rwork	0.198
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011 *
RMSD bond angle	1.660 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.070 *	0.235 *
Number of reflections	16619
Completeness [%]	92.6	90.6
Redundancy	2. *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8 *	20 *	1.2M ammonium sulfate, 0.1M sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.2 (mg/ml)
2	1	reservoir	PEG1000	12 (%)
3	1	reservoir	imidazole-malate	0.1 (M)	pH5.8