1LCW

streptavidin-homobiotin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	297
Detector technology	IMAGE PLATE
Collection date	1998-07-17
Detector	RIGAKU RAXIS IIC
Spacegroup name	I 2 2 2
Unit cell lengths	47.320, 95.760, 105.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.183
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.270 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.138 *	0.328 *
Number of reflections	11861
Completeness [%]	94.3	98.6
Redundancy	2. *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8 *	20 *	ammonium sulfate, sodium acetate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.2 (mg/ml)
2	1	reservoir	PEG1000	12 (%)
3	1	reservoir	imidazole-malate	0.1 (M)	pH5.8