1LCU
Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-04 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.456, 103.029, 126.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 3.500 |
| R-factor | 0.193 * |
| Rwork | 0.196 |
| R-free | 0.26600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1esv |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.390 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.115 | 0.249 * |
| Total number of observations | 101490 * | |
| Number of reflections | 15407 | |
| Completeness [%] | 88.8 | 88.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.7 * | 4 * | Ammonium sulfate, MgCl2, Imidazole, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | actin | 0.240 (mM) | |
| 2 | 1 | drop | polylysine | 0.120 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.3 (M) | |
| 4 | 1 | reservoir | ATP | 1.0 (mM) | |
| 5 | 1 | reservoir | 3.0 (mM) | ||
| 6 | 1 | reservoir | imidazole | 60 (mM) | pH6.7 |
