1LCK
SH3-SH2 DOMAIN FRAGMENT OF HUMAN P56-LCK TYROSINE KINASE COMPLEXED WITH THE 10 RESIDUE SYNTHETIC PHOSPHOTYROSYL PEPTIDE TEGQPYQPQPA
Experimental procedure
| Spacegroup name | H 3 2 |
| Unit cell lengths | 72.350, 72.350, 187.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 5.000 * - 2.500 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.32900 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | Tris | 50 (mM) | |
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | sodium azide | 0.02 (%) | |
| 6 | 1 | drop | phosphopeptide | 6 (mM) | |
| 7 | 1 | reservoir | sodium tartrate | 950 (mM) | |
| 8 | 1 | reservoir | ammonium sulphate | 400 (mM) | |
| 9 | 1 | reservoir | Tris | 100 (mM) |
