1LCE
LACTOBACILLUS CASEI THYMIDYLATE SYNTHASE TERNARY COMPLEX WITH DUMP AND CH2THF
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-08-18 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 78.900, 78.900, 229.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 26.900 * |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.800 |
| High resolution limit [Å] | 2.430 |
| Rmerge | 0.098 |
| Number of reflections | 9305 |
| Completeness [%] | 55.0 |
| Redundancy | 1.818 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | Hardy, L.W., (1987) Science, 235, 448. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | thymidylate synthase | 10 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 20 (mM) | |
| 3 | 1 | reservoir | potassium phosphate | 20 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 15-20 (mM) | |
| 5 | 1 | reservoir | dUMP | 5 (mM) | with or without |
