1LC7
Crystal Structure of L-Threonine-O-3-phosphate Decarboxylase from S. enterica complexed with a substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.9763 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.280, 103.860, 117.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 1.860 - 1.800 |
| Rwork | 0.212 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1KUS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.530 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.219 |
| Number of reflections | 38108 | |
| <I/σ(I)> | 60.8 | 10.2 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6 | 4 * | used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | L-threonine phosphate | 50 (mM) | |
| 2 | 1 | 1 | protein | 9 (mg/ml) | |
| 3 | 1 | 1 | PEG2000ME | 10 (%) | |
| 4 | 1 | 1 | sodium potassium phosphate | 100 (mM) | |
| 5 | 1 | 1 | MES | 100 (mM) | pH6.0 |
