1LC3
Crystal Structure of a Biliverdin Reductase Enzyme-Cofactor Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.780, 76.140, 75.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.500 |
| Rwork | 0.214 |
| R-free | 0.23800 * |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.072 * | 0.389 * |
| Total number of observations | 131242 * | |
| Number of reflections | 47157 | |
| Completeness [%] | 97.2 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 4 * | 2.0 M Na+/K+ phosphate, 100 mM CAPS, 200 mM lithium sulfate (Emerald Screen Wizard-I condition 27), pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.5 (mg/ml) | |
| 2 | 1 | reservoir | sodium potassium phosphate | 2.0 (M) | |
| 3 | 1 | reservoir | CAPS | 100 (mM) | pH10.5 |
| 4 | 1 | reservoir | lithium sulfate | 200 (mM) |
