1LBG

LACTOSE OPERON REPRESSOR BOUND TO 21-BASE PAIR SYMMETRIC OPERATOR DNA, ALPHA CARBONS ONLY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Detector technology	AREA DETECTOR
Detector	SIEMENS
Spacegroup name	C 1 2 1
Unit cell lengths	104.300, 224.400, 112.100
Unit cell angles	90.00, 95.70, 90.00

Refinement procedure

Resolution	15.000 - 4.800
R-factor	0.26
Rwork	0.260
RMSD bond length	0.011
RMSD bond angle	1.800
Data reduction software	XDS
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	4.800 *
Rmerge	0.058
Total number of observations	43976 *
Number of reflections	11283
Completeness [%]	90.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	macro seeding *	8.5 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG3000	32 (%)
2	1	drop	potassium phosphate	0.05 (M)
3	1	drop	sodium potassium tartrate	0.075 (M)
4	1	drop	protein	0.055 (mM)
5	1	drop	DNA	0.194 (mM)
6	1	reservoir	PEG3000	29-31 (%)
7	1	reservoir	potassium phosphate	0.05 (M)
8	1	reservoir	sodium potassium tartrate	0.075 (M)