1LB6

TRAF6-CD40 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-21
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	39.879, 43.815, 101.435
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.800
Rwork	0.203
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRAF6 apo form
RMSD bond length	0.014 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.056 *	0.221 *
Number of reflections	15569
Completeness [%]	99.1 *	93 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5	293	PEG8000, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	5-25 (%)
2	1	1	Tris-HCl	100 (mM)	pH7.5