1LB5

TRAF6-RANK Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	1999-09-21
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	37.990, 44.991, 106.535
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.000 *
Rwork	0.213
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TRAF6 apo form
RMSD bond length	0.005
RMSD bond angle	1.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.550
High resolution limit [Å]	2.000 *	2.400
Rmerge	0.055 *	0.139 *
Number of reflections	12396
Completeness [%]	96.0	86.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5	293	PEG8000, Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	5-25 (%)
2	1	1	Tris-HCl	100 (mM)	pH7.5