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CRYSTAL STRUCTURE OF CYTOMEGALOVIRUS PROTEASE

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Detector technology	CCD
Collection date	1996-02-10
Spacegroup name	P 43 2 2
Unit cell lengths	58.700, 58.700, 131.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.500
R-factor	0.185
Rwork	0.185
RMSD bond length	0.016
RMSD bond angle	2.200
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.060
Total number of observations	22880 *
Number of reflections	7644
Completeness [%]	90.0
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG400	30 (%)
2	1	reservoir	phosphate citrate buffer	0.1 (M)
3	1	drop	protease	3.5 (mg/ml)
4	1	drop	PEG400	15 (%)
5	1	drop	phosphate citrate buffer	0.05 (M)