1LAW

STABILIZATION OF ESCHERICHIA COLI RIBONUCLEASE HI BY CAVITY-FILLING MUTATIONS WITHIN A HYDROPHOBIC CORE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	44.060, 86.840, 35.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.188
RMSD bond length	0.015
RMSD bond angle	0.034
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	reservoir	Tris-HCl	200 (mM)