1LAR

CRYSTAL STRUCTURE OF THE TANDEM PHOSPHATASE DOMAINS OF RPTP LAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	113
Detector technology	CCD
Collection date	1997-12
Spacegroup name	P 1 21 1
Unit cell lengths	66.920, 62.730, 161.590
Unit cell angles	90.00, 98.94, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.222 *
Rwork	0.222
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yfo
RMSD bond length	0.007
RMSD bond angle	1.330
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Rmerge	0.064
Total number of observations	267242 *
Number of reflections	81312
<I/σ(I)>	7
Completeness [%]	91.0
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	100 (mM)
3	1	drop	PEG8000	8-16 (%(w/v))
4	1	drop		0.2 (M)