1LAN

LEUCINE AMINOPEPTIDASE COMPLEX WITH L-LEUCINAL

Experimental procedure

Temperature [K]	123
Detector technology	AREA DETECTOR
Collection date	1995-06-06
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	P 63 2 2
Unit cell lengths	131.200, 131.200, 120.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 1.900
R-factor	0.168
Rwork	0.168
R-free	0.21200
RMSD bond length	0.010
RMSD bond angle	1.700 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.070
Total number of observations	160257 *
Number of reflections	44837
Completeness [%]	99.3
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8		pH 7.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	b1LAP	7 (mg/ml)
2	1	drop		0.050 (mM)
3	1	drop		200 (mM)
4	1	drop	Tris-HCl	50 (mM)
5	1	reservoir	Tris-HCl	50 (mM)
6	1	reservoir		0.050 (mM)